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目的探讨苯并咪唑衍生物BMT-1对急性B淋巴母细胞瘤细胞增殖活力的影响及其相关机制。方法采用CCK-8方法测定BMT-1对Daudi、Nalm-6的细胞毒性;采用流式细胞技术方法检测BMT-1对Daudi细胞凋亡及线粒体膜电位的影响;采用Western Blot方法检测Daudi细胞凋亡相关蛋白PARP的表达情况。结果BMT-1对Daudi、Nalm-6细胞具有较强的细胞毒性;BMT-1可诱导Daudi细胞凋亡,引起Daudi细胞线粒体膜电位下降,并可使得Daudi细胞产生PARP蛋白剪切体。结论 BMT-1对B淋巴母细胞瘤具有细胞毒性作用,其关键机制为诱导细胞凋亡和引起线粒体膜电位下降,为BMT-1治疗急性淋巴细胞白血病提供了理论基础。
Objective To investigate the effect of benzimidazole derivative BMT-1 on the proliferation of acute B lymphoblastoma cells and its related mechanisms. Methods The cytotoxicity of BMT-1 to Daudi and Nalm-6 was determined by CCK-8 assay. The effect of BMT-1 on apoptosis and mitochondrial membrane potential of Daudi cells was detected by flow cytometry. The apoptosis of Daudi cells was detected by Western Blot PARP protein expression. Results BMT-1 was highly cytotoxic to Daudi and Nalm-6 cells. BMT-1 induced apoptosis in Daudi cells and decreased the mitochondrial membrane potential in Daudi cells, and produced PARP protein splicing in Daudi cells. Conclusions BMT-1 has a cytotoxic effect on B-cell lymphoblastoma. The key mechanism is to induce apoptosis and decrease the mitochondrial membrane potential, which provides a theoretical basis for the treatment of acute lymphoblastic leukemia with BMT-1.