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利用傅立叶交换显微红外光谱法(Microscopic Fourier Transform Infrared Spectroscopy,Micro-FTIR),对50份鼻咽癌组织及相应正常组织的石蜡切片进行检测、分析。结果显示,癌变组织与正常组织红外谱图中蛋白质酰胺Ⅰ带、酰胺Ⅱ带、磷酸二酯基团、糖原等的吸收峰不仅在位置上存在明显差别,而且癌变组织与正常组织相比,1081cm~(-1)处磷酸二酯基团的吸收峰在强度上增加了1.77±0.23倍,从侧面反映了细胞癌变过程中细胞无限增殖的特性。所以显微红外光谱法可在分子水平上将癌组织与正常组织区分开。
The paraffin sections of 50 nasopharyngeal carcinoma tissues and corresponding normal tissues were detected and analyzed by using Fourier transform infrared microscopy (Micro-FTIR). The results showed that the absorption peak of protein amide Ⅰ band, amide Ⅱ band, phosphodiester group, glycogen, etc. in the infrared spectrum of cancerous tissues and normal tissues not only had obvious difference in the position, but also, compared with the normal tissues, The absorption peak of the phosphodiester group at 1081 cm -1 increased by 1.77 ± 0.23 fold, which reflected the immortal cell proliferation in the process of carcinogenesis. Therefore, microscopic infrared spectroscopy can distinguish cancerous tissue from normal tissue at the molecular level.