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目的 建立胃癌下调基因组 c DNA抑制消减文库并鉴定。方法 采用高灵敏度的抑制消减杂交技术 ,建立五人份正常胃粘膜 m RNA(Tester)抑制消减杂交胃癌 m RNA(Driver)的差异表达文库 ,用PCR及差异表达克隆菌转膜反向杂交判定其消减效率。结果 所构建胃癌下调基因抑制消减杂交 c DNA文库 ,消减效率高 ,胃癌下调基因在正常胃粘膜及胃癌组织中的差异表达符合率达 86 %。结论 成功建立了用于筛选胃癌下调基因的抑制消减杂交 c DNA文库。
Objective To establish a gastric cancer down-regulated genomic c DNA suppression subtractive library and identification. Methods A high-sensitivity suppression subtractive hybridization (SSH) technique was used to establish a differential expression library in which five human normal gastric mucosa m RNAs (Tester) Reduce efficiency. Results The down-regulated gene of gastric cancer suppresses the hybridization of c-DNA library, and the efficiency of abatement is high. The coincidence rate of down-regulated genes in gastric mucosa and gastric cancer tissues is 86%. Conclusion The suppression subtractive hybridization (cDNA) library was successfully established for the screening of gastric cancer down-regulated genes.