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目的:考察补骨脂中苯并呋喃苷类化合物(补骨脂苷、异补骨脂苷)在大鼠血浆的移行特征。方法:大鼠灌胃给予苯并呋喃苷部位提取物,采用HPLC-UV测定大鼠血浆中补骨脂苷、异补骨脂苷的含量,流动相0.1%甲酸水溶液-乙腈梯度洗脱,检测波长246 nm,血药浓度-时间数据采运用SPSS 11.0软件分析。结果:补骨脂苷和异补骨脂苷在15 min后在血液中可检测到,于1.5 h均达峰浓度,苯并呋喃苷类成分在大鼠体内吸收较快,补骨脂苷、异补骨脂苷在大鼠体内能转化为补骨脂素、异补骨脂素。结论:苯并呋喃苷类成分血浆HPLC特征性及专属性好,适用于该部位血浆移行成分的分析,为补骨脂相关制剂的临床前药动学研究提供参考。
Objective: To investigate the migration characteristics of benzofuranosides (psoralen and isopsoralen) in plasma of rats. Methods: The rats were intragastrically given benzofuranoside extract, using HPLC-UV determination of psoralen, isopsoralen content in the plasma, mobile phase 0.1% formic acid in water - acetonitrile gradient elution, detection Wavelength 246 nm, plasma concentration - time data mining using SPSS 11.0 software analysis. Results: Psoralen and isopsoralen could be detected in the blood after 15 min, reached the peak concentration at 1.5 h, and benzofuranoside absorbed rapidly in rats. Psoralen, Isopsoralen in rats can be transformed into psoralen, isopsoralen. CONCLUSION: The HPLC of benzofuranoside is characterized by its high specificity and specificity. It is suitable for the analysis of plasma components in this area and provides a reference for the preclinical pharmacokinetics of psoralen-related agents.