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本文研究了用30KevN+离子束注入大麦干种子后其M1代的细胞生物学效应。研究结果表明,低剂量的N+离子注入对大麦种子的萌发及M1胚根、胚芽细胞的有丝分裂有明显的促进作用。离子注入均能诱发胚根细胞和胚芽细胞的染色体畸变和核畸变,呈现微核、双核、小核、桥、断片和落后染色体等多种类型。并在2×1016N+/cm2-8×1016N+/cm2剂量范围内,注入剂量与畸变率之间有显著的正相关,但到1×1017N+/cm2后畸变率却反而下降。研究结果还显示胚芽细胞较胚根细胞对氮离子束更为敏感。
In this paper, the cell biology effects of M1 generation after the seeds of barley were injected with 30Kev N + ion beam were studied. The results showed that the low dose of N + ion implantation significantly promoted the germination of barley seeds and the mitosis of M1 radicle and germ cells. Both ion implantation can induce chromosome aberration and nuclear aberration in radicle cells and embryo cells, showing micronuclei, binuclear, small nuclei, bridges, fragments and backward chromosomes. In the dose range of 2 × 10 16 N + / cm 2 -8 × 10 16 N + / cm 2, there was a significant positive correlation between the dose and the distortion rate, but the distortion rate decreased to 1 × 10 17 N + / cm 2. The results also show that germ cells are more sensitive to nitrogen ion beams than radicle cells.