小肠结肠炎耶尔森氏菌可引起肠道内外多种感染,其血清型达50多种且与致病性有关。作者等用PCR 扩增y、c 染色体上163bp 的yst 基因。选用一对引物,可扩增几乎整个yst 基因的213核苷酸,但鉴别致病性与非致病性的y、c 的特异性不强。经选用另一对引物扩增yst 基因的特异部分,通过复式在 Yersinia enterocolitis can cause a variety of infections both inside and outside the intestine, the serotypes of more than 50 kinds and with pathogenicity. The authors used PCR to amplify the 163bp yst gene on y and c chromosomes. A pair of primers was used to amplify nearly 213 nucleotides of the entire yst gene. However, the specificity of distinguishing pathogenic and non-pathogenic y and c was not strong. Another pair of primers was used to amplify the specific portion of the yst gene, by multiple additions