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目的 :以原发性喉癌患者为研究对象 ,检测喉癌组织中P16抑癌基因纯合缺失和异常甲基化情况。方法 :采用PCR、多重PCR、限制性内切酶解 PCR方法对 2 5例喉癌患者和 2 5例喉部非恶性肿瘤 (息肉、慢性炎症 )患者 ,进行P16基因纯合缺失和异常甲基化检测。结果 :2 5例喉癌患者纯合缺失 2 0 %( 5 / 2 5 ) ,异常甲基化 2 4%( 6/ 2 5 ) ,2 5例非恶性肿瘤患者纯合缺失未检出 ( 0 / 3 4) ,异常甲基化 8( 2 / 2 5 )。结论 :本研究喉癌患者P16抑癌基因总失活率 44 %( 11/ 2 5 ) ,表明P16基因失活在喉癌患者中是较为常见的基因变化。喉癌患者中甲基化异常是P16基因失活的另一重要途径 ,值得引起重视
Objective: To investigate the homozygous deletion and abnormal methylation of P16 tumor suppressor gene in laryngeal cancer patients with primary laryngeal cancer patients. Methods: Twenty-five patients with laryngeal cancer and 25 non-malignant tumors of the larynx (polyps, chronic inflammation) were subjected to PCR, multiplex PCR and restriction endonuclease digestion (PCR). The homozygous deletion of P16 gene and abnormal methyl Detection. Results: Homozygous deletion was 20% (5/25), abnormal methylation was 24% (6/25) in 25 cases of laryngeal cancer, and homozygous deletion was not detected in 25 cases of non-malignant tumor / 3 4), abnormal methylation 8 (2/2 5). Conclusion: The total inactivation rate of P16 tumor suppressor gene in this study was 44% (11/2 5), indicating that the inactivation of P16 gene is a common genetic change in patients with laryngeal cancer. Methylation abnormalities in patients with laryngeal carcinoma is another important way inactivation of P16 gene, it deserves attention