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目的:探讨p15基因纯合性缺失和血管内皮生长因子(vascularendothelialgrowthfactor,VEGF)过表达的相关性及两者与非小细胞肺癌(non-smallcelllungcancer,NSCLC)生物学特征之间的关系。方法:提取DNA,采用PCR技术,分别对97例NSCLC组织和20例癌旁正常组织进行p15基因纯合缺失检测;提取组织RNA,采用RT-PCR技术分析VEGF基因表达水平。结果:NSCLC组织中p15基因纯合缺失率和VEGF表达率分别为44.3%(43/97)和66.0%(64/97),显著高于癌旁正常组织的5%(1/20)和10%(2/20),P<0·05。p15基因纯合缺失与TNM分期(P=0·001)和淋巴结转移相关(P=0·018),而与组织学类型无相关性,P=5·731;VEGF过表达与TNM分期和淋巴结转移相关,P值均<0·01;而与组织学类型无相关性,P=2·113。p15基因纯合缺失与VEGF表达呈正相关。结论:p15基因纯合缺失和VEGF基因过表达可能在NSCLC的发生及恶性进展中发挥一定的作用。
Objective: To investigate the relationship between the loss of homozygosity of p15 gene and the overexpression of vascular endothelial growth factor (VEGF) and their biological characteristics in non-small cell lung cancer (NSCLC). METHODS: DNA was extracted and the homozygous deletion of p15 gene was detected in 97 NSCLC tissues and 20 adjacent normal tissues by PCR. The RNA was extracted and the VEGF gene expression was analyzed by RT-PCR. Results: The homozygous deletion rate and VEGF expression rate of p15 gene in NSCLC tissues were 44.3% (43/97) and 66.0% (64/97), respectively, which were significantly higher than those in 5% (1/20) and 10 % (2/20), P <0 · 05. The homozygous deletion of p15 gene was associated with TNM stage (P = 0.001) and lymph node metastasis (P = 0.018), but not with histological type (P = 5.73 1). VEGF overexpression correlated with TNM stage and lymph node Metastasis, P values were <0.01; but no correlation with histological type, P = 2.113. Homozygous deletion of p15 gene was positively correlated with VEGF expression. Conclusion: Loss of homozygosity and overexpression of VEGF gene in p15 gene may play a role in the occurrence and malignant progression of NSCLC.