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目的:观察Orexin受体1对摄食条件反射的调控研究。方法:将40只大鼠随机分为四组,分别为NS/NS组,SB/SB组,NS/SB组,SB/NS组,每组10只大鼠,给予大鼠八组“条件刺激-无条件刺激(CS-US)”训练,给予无条件刺激后立即进行条件刺激,每组进行训练前30 min给予SB或生理盐水(NS)。获得性训练后,给予2组反射消失性训练,即给予8次条件刺激。条件刺激是给予大鼠10 s,2kHZ声音刺激,无条件刺激是直接给予大鼠食物。结果:给予条件刺激后,四组大鼠摄食行为均明显增加,摄食间隔均明显缩短,当条件刺激强度增加时,摄食行为也增加,而预先给予SB,与NS/NS组相比,其余组大鼠摄食行为相对减少(P<0.05),摄食间隔增加。消失性训练中,与NS/NS,NS/SB组相比,SB/NS组和SB/SB组大鼠摄食行为明显减少(P<0.05)。与其他三组大鼠相比,SB/NS组大鼠摄食间隔缩短。预先给予SB使后两次刺激后摄食间隔明显增加(P<0.05)。结论:OX1R信号通路调控摄食反射的产生和消失。
OBJECTIVE: To observe the regulation of Orexin receptor 1 on the regulation of food intake. Methods: Forty rats were randomly divided into four groups: NS / NS group, SB / SB group, NS / SB group and SB / NS group, with 10 rats in each group. Stimulus-Unconditional Stimulation (CS-US) "training, given unconditional stimuli immediately after the conditions of stimulation, 30 minutes before training each group given SB or saline (NS). After acquired training, two groups of reflexive training were given, that is, eight conditioned stimuli were given. Conditional stimulation is given to rats 10 s, 2kHZ sound stimulation, unconditional stimulation is given directly to the rat food. Results: After the stimulation, the feeding behavior of the four groups were significantly increased, the feeding interval was significantly shortened, when the stimulus intensity increased, the feeding behavior also increased, and pre-given SB, compared with the NS / NS group, the remaining group Feeding behavior of rats was relatively decreased (P <0.05), feeding interval increased. Compared with NS / NS and NS / SB groups, feeding behavior of rats in SB / NS group and SB / SB group was significantly decreased (P <0.05) in vanishing training. Compared with the other three groups of rats, SB / NS rats feeding interval shortening. Pretreatment with SB resulted in a significant increase in feeding interval after the two stimuli (P <0.05). Conclusion: OX1R signaling pathway regulates the production and disappearance of food reflex.