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以“艾西丝”南瓜组培苗为材料，研究其在诱导不定根形成过程中过氧化物酶活性及同工酶谱的变化。结果表明，当南瓜无根苗从含有较高浓度的ＢＡ培养基转入１／２ＭＳ培养基后，可诱导无根苗茎基部不定根生成，一般在转入生根培养基第３ｄ开始生根，至第６ｄ生根率达７０％以上。在此期间，南瓜茎内过氧化物酶活性由低增高，即在不定根形成之前，过氧化物酶活性处在较低水平，而当不定根形成时，过氧化物酶活性迅速升高，以后一直维持在较高水平；同时过氧化物酶的同工酶谱也发生了变化，在不定根形成前酶带为３条，Ｒｆ分别为０．４４，０．５８，０．６６。而在不定根开始形成时及形成以后，酶带增至４条，即在阴极区增加了Ｒｆ为０．３５的谱带。这反映出在南瓜不定根形成各个阶段有不同同工酶在起作用。
In this study, the tissue culture of pumpkin washers was used to study the changes of peroxidase activity and isozymes during the induction of adventitious root formation. The results showed that adventitious root formation at the base of rootless seedlings could be induced when the rootless seedlings of pumpkin were transformed into 1/2 MS medium with higher concentration of BA, Rate of more than 70%. During this period, the pumpkin stem peroxidase activity increased from low, that is in the adventitious root formation before the peroxidase activity at a lower level, and when adventitious root formation, peroxidase activity increased rapidly, has been since Maintained at a relatively high level. At the same time, the isozymes of peroxidase also changed. There were 3 bands before adventitious root formation, with Rf of 0.44, 0.58 and 0.66 respectively. However, when adventitious roots started to form and formed, the number of bands increased to 4, that is, an increase of Rf of 0.35 in the cathode region was observed. This reflects the role of different isoenzymes during various stages of adventitious root formation in pumpkin.