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目的 观察血管紧张素Ⅱ受体 1拮抗剂洛沙坦和血管紧张素转换酶抑制剂苯那普利对肾病大鼠的早期保护作用。方法 16只大鼠均分 4组 :(1)正常对照组 ;(2 )苯那普利组 ;(3)洛沙坦组 ;(4 )非治疗组。对大鼠单侧肾切除 1周后行阿霉素 (5mg/kg)注射 ,建立动物模型。自手术后分别给予苯那普利 6mg/ (kg·d)或洛沙坦 5mg/ (kg·d)治疗 5周 ,观察大鼠的蛋白尿 ,血总蛋白、白蛋白、肌酐、尿素氮、胆固醇和残余肾组织的病理改变。结果 实验 3周时 ,正常对照组、苯那普利组、洛沙坦组及非治疗组大鼠尿蛋白依次 (下同 )为 2 6 (1 3～ 5 6 )mg/ 2 4h、130 3(10 0 9～ 176 8)mg/ 2 4h、42 7(4 1 9～ 44 2 )mg/ 2 4h和 10 5 8(85 1～ 144 9)mg/ 2 4h (H =11 6 5 ,P <0 0 1)。实验 5周 (结束 )时四组大鼠尿蛋白分别为 4 4(0 7～ 5 8)mg/ 2 4h、5 4 8(32 8～ 72 0 )mg/ 2 4h、2 6 7(2 5 6～ 2 8 1)mg/ 2 4h和43 2 (31 6～ 5 0 9)mg/ 2 4h (H =11 30 ,P <0 0 1) ;血胆固醇分别为 (1 9± 0 3)mmol/L、(5 0± 1 6 )mmol/L、(4 4± 1 4)mmol/L和 (8 7± 2 4)mmol/L (F =11 2 6 ,P <0 0 1) ;肾小球系膜增生率分别为7 3(1 4～ 12 8) %、30 3(8 2～ 6 4 5 ) %、32 8(6 0～ 5 5 0 ) %和 6 5 7(2
Objective To observe the early protective effects of losartan and angiotensin converting enzyme inhibitor benazapril, an angiotensin Ⅱ receptor antagonist, on nephrotic rats. Methods 16 rats were divided into 4 groups: (1) normal control group; (2) benazepril group; (3) losartan group; (4) non-treatment group. One week after unilateral nephrectomy in rats, doxorubicin (5 mg / kg) was injected to establish an animal model. The rats were treated with benazepril 6mg / (kg · d) or losartan 5mg / (kg · d) for 5 weeks after operation. Proteinuria, serum total protein, albumin, creatinine, urea nitrogen, Cholesterol and residual kidney tissue pathological changes. Results At the 3rd week of experiment, urinary protein in normal control group, benazepril group, losartan group and untreated group were 26 (1 3 ~ 56) mg / 2 4h, 130 3 (10 9 ~ 176 8) mg / 2 4h, 42 7 (4 1 9 ~ 44 2) mg / 2 4h and 10 5 8 (85 1 ~ 144 9) mg / 2 4h <0 0 1). At the end of the experiment, the urinary protein in the four groups was 44 (0 7 ~ 58) mg / 2 4h, 548 (32 8 ~ 72 0) mg / 2 4h, 2 6 7 (2 5 6 2981 and 43 2 31 6 ~ 50 9 mg / 2 4 h respectively (H = 11 30, P 0 01). The levels of blood cholesterol were (1 9 ± 0 3) mmol / (50 ± 1 6) mmol / L, (44 ± 1 4) mmol / L and (87 ± 2 4) mmol / L The mesangial proliferative rates of small globules were 73% (14 ~ 12%), 30 3 (82% ~ 545%), 32 8% (60% ~ 65%) and 657%