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目的对柽柳(Tamarix chinensis Lour.)抗肿瘤活性成分进行分离和鉴定。方法使用硅胶、SephadexLH-20、ODS和HPLC分离、纯化,利用NMR技术确定结构。采用MTT法和SRB法测定了所得化合物的抗肿瘤活性。结果分离鉴定了6个甾体类化合物和7个黄酮类化合物,分别为β-谷甾醇(β-sitosterol,1)、豆甾-4-烯-3,6-二酮(stigmast-4-ene-3,6-dione,2)、麦角甾-4,24(28)-二烯-3-酮[ergosta-4,24(28)-diene-3-one,3]、豆甾烷-3,6-二酮(stigmast-4-ene-3,6-dione,4)、豆甾-4-烯-3-酮(sitosterone,5)、胆甾醇(cholesterol,6)、5-羟基-7,4′-二甲氧基黄酮(5-hydroxy-7,4′-dimethoxyflavone,7)、山柰酚(kaempferol,8)、山柰酚-4′-甲醚(4′-methylkaempfero,9)、山柰酚-7-甲醚(7-methylkaempfero,10)、山柰酚-4′,7-二甲醚(4′,7-dimethylkaempfero,11)、槲皮素(quercetin,12)、槲皮素-3′,4′-二甲醚(3′,4′-dimethylquercetin,13)。结论化合物2,3,4,5和7首次从柽柳属中分离得到。体外抗肿瘤活性实验显示,在50mg·L-1下,化合物2,3,4和5对人肺癌细胞A-549显示有较强的细胞毒活性。
Objective To isolate and identify the antitumor active ingredients of Tamarix chinensis Lour. The method was isolated and purified using silica gel, Sephadex LH-20, ODS and HPLC, and the structure was confirmed by NMR. The antitumor activity of the obtained compounds was determined by MTT method and SRB method. Results Six steroidal compounds and seven flavonoids were isolated and identified as β-sitosterol (1), stigmast-4-ene -3,6-dione, 2) ergosta-4,24 (28) -dien-3-one [ergosta-4,24 (28) -diene-3-one, , Stigmast-4-ene-3,6-dione (4), sitosterone (5), cholesterol, , 4’-dimethoxyflavone (7), kaempferol (8), 4’-methylkaempfero (9) , 7-methylkaempfero (10), kaempferol-4 ’, 7-dimethylkaempfero, quercetin (12), quercetin 3 ’, 4’-dimethylquercetin (13). Conclusion Compounds 2,3,4,5 and 7 were isolated from Tamarix for the first time. In vitro antitumor activity experiments showed that compounds 2, 3, 4 and 5 showed strong cytotoxic activity against A-549 cells at 50 mg · L -1.