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[目的]分离纯化浙江蝮蛇蛇毒主要组分并分析其对抑制白血病细胞株K562的细胞增殖作用。[方法]采用DEAE-Cellulose、CM-Sephadex C-50和Sephadex G-75柱层析,以离子交换和凝胶过滤法从蝮蛇蛇毒中分离纯化主要组分;SDS-PAGE检测其纯度和估计其分子量;MTT及FCM方法检测蛇毒主要组分对白血病细胞的增殖抑制。[结果]从浙江蝮蛇蛇毒中分离纯化得到一分子量约为30kD组分,该组分对白血病细胞具有较强的细胞增殖抑制作用,且呈剂量依赖性关系,5.0、10.0mmol/L浓度时对K562细胞增殖抑制率分别达26.32%和32.28%,并使细胞阻滞于G2/M期。[结论]浙江蝮蛇蛇毒可抑制白血病细胞的增殖,其有较好的应用前景。
[Objective] The aim was to isolate and purify the main components of the snake venom from Zhejiang and analyze its effect on inhibiting the proliferation of K562 leukemia cells. [Method] The main constituents were isolated and purified from the snake venom snake venom by ion exchange and gel filtration using DEAE-Cellulose, CM-Sephadex C-50 and Sephadex G-75 column chromatography. Purity and estimated by SDS-PAGE Its molecular weight; MTT and FCM method to detect the main components of snake venom for inhibiting the proliferation of leukemia cells. [Result] A component of about 30kD molecular weight was isolated and purified from the snake venom of Agkistrodon acutus, which had a strong inhibitory effect on the proliferation of leukemia cells in a dose - dependent manner at the concentration of 5.0 and 10.0mmol / L The inhibitory rates of proliferation on K562 cells were 26.32% and 32.28%, respectively, and the cells were arrested at G2 / M phase. [Conclusion] Agkistrodon acutus venom can inhibit the proliferation of leukemia cells, which has a good application prospect.