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目的:建立肾虚黄体抑制流产大鼠模型,从细胞因子角度探讨模型大鼠流产机理,观察中药的干预作用,并进一步探讨其作用机制。方法:以羟基脲加米非司酮建立肾虚黄体抑制流产大鼠模型,观察比较正常妊娠组、助孕3号方组、黄体酮组、模型组大鼠孕第11天血清IL(白细胞介素)-2、IL-10、P (孕激素)及NP(一氧化氮)含量,计算胚胎吸收率。结果:除正常妊娠组外,其余各组大鼠使用羟基脲后均有不同程度的毛松、毛干枯、倦卧、活动力下降、纳食减少、大便稀烂、体重减轻等肾虚症状。助孕3号方组体重减轻程度较模型组轻微,差异有显著性意义(P<0.05),而黄体酮组体重减轻程度与模型组比较,差异无显著性意义(P>0.05)。与模型组比较.正常妊娠组、助孕3号方组大鼠血清IL-2水平、IL-2/IL-10比值明显降低(P<0.05)。与模型组比较,助孕3号方组、黄体酮组及正常妊娠组血清P含量均明显升高(P<0.05),血清NO含量均明显降低(P<0.05)。与模型组比较,助孕3号方组、黄体酮组、正常妊娠组大鼠胚胎吸收率均明显下降(P<0.05)。而助孕3号方组、黄体酮组、正常妊娠组间胚胎吸收率比较,差异均无显著性意义(P>0.05)。结论:助孕3号方能够通过抑制Th(T细胞辅助)1型细胞因子表达,抑制NO,促进P分泌,多环节共同调节Th1/Th2型细胞因子间的平衡.发挥其保胎作用。
OBJECTIVE: To establish a rat model of inhibiting abortion with kidney deficiency and luteinizing, and to investigate the mechanism of abortion in model rats from the perspective of cytokines, observe the intervention of traditional Chinese medicine, and further explore its mechanism of action. METHODS: A rat model of abortion was established by using hydroxyurea plus mifepristone to inhibit the luteal body of kidney deficiency. The levels of serum IL (interleukin) at the 11th day of pregnancy were compared between normal pregnant group, Zhuyun 3 group, progesterone group and model group rats. )-2, IL-10, P (progestin) and NP (nitric oxide) content, embryonic uptake rate was calculated. Results: In addition to the normal pregnancy group, the remaining rats in each group had different degrees of hairy pine, dry hair, tiredness, decreased mobility, reduced food intake, loose stools, and weight loss. The degree of weight loss in the Zhuyun 3 group was milder than that in the model group (P<0.05). There was no significant difference in weight loss between the progesterone group and the model group (P>0.05). Compared with the model group, the serum IL-2 level and IL-2/IL-10 ratio in the normal pregnant group and Zhuyun 3 group were significantly lower (P<0.05). Compared with the model group, the serum P content in the Zhuyun 3 group, the progesterone group and the normal pregnancy group was significantly increased (P<0.05), and the serum NO content was significantly reduced (P<0.05). Compared with the model group, the rate of embryonic uptake in the Zhuyun 3 group, the progesterone group, and the normal pregnant group decreased significantly (P<0.05). However, there was no significant difference in the rate of embryonic uptake between Zhuyun 3 group, progesterone group and normal pregnant group (P>0.05). Conclusion: Zhuyun 3 can inhibit the expression of type 1 cytokines of Th (T cell helper), inhibit NO, and promote the secretion of P. It can regulate the balance between Th1/Th2 cytokines and play a role in the prevention of miscarriage.