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目的：探讨日本血吸虫２６ｋＤａ基因重组蛋白（ｒＳｊｃ２６ＧＳＴ）抗日本血吸虫生殖作用及其免疫机制。方法：小鼠经ｒＳｊｃ２６ＧＳＴ免疫，攻击感染日本血吸虫尾蚴４０±１条，于６ｗｋ后解剖检虫体，计算虫数与肝组织和脾组织内的虫卵数，并对虫体主要生殖器官作透射电镜超微结构的观察。结果：证明ｒＳｊｃ２６ＧＳＴ具有明确的抗生殖免疫作用。与对照组相比，免疫鼠减虫率为３０．０％，肝组织和脾组织内虫卵减少率分别为５７．１％与７９．９％，透射电镜观察显示免疫鼠体内雌虫的卵黄腺及雄虫的睾丸组织均产生不同程度的抑制作用，主要表现为卵黄细胞胞质中卵黄球、卵黄滴、脂滴和睾丸组织中精细胞、支持细胞胞质中的脂滴数量均有明显减少。结论：ｒＳｊｃ２６ＧＳＴ有抗日本血吸虫生殖的作用，使虫体生殖器官受到损害
Objective: To investigate the anti-Schistosoma japonicum reproductive effect and its immune mechanism of Schistosoma japonicum 26kDa recombinant protein (rSjc26GST). Methods: The mice were immunized with rSjc26GST and challenged with 40 ± 1 S. japonicum cercariae after 6 wk. The number of insects and the number of eggs in liver and spleen tissues were calculated and transmitted to the main reproductive organs Observation of ultrastructure of electron microscope. Results: Proved rSjc26GST has a clear anti-reproductive effect. Compared with the control group, the worm reduction rate of the immunized mice was 30.0%, the reduction rates of the eggs in the liver and spleen tissues were 57.1% and 79.9%, respectively. The transmission electron microscope showed that the yolk The testes of gland and male had different degrees of inhibition. The main manifestations were the yolk ball, the egg yolk drop, the lipid droplet and the spermatid in testis tissue in the cytoplasm of the yolk and the number of lipid droplets in the supporting cells was obvious cut back. Conclusion: rSjc26GST has anti-Schistosoma japonicum reproductive effects, so that the reproductive organs of insect damage