论文部分内容阅读
目的:观察半枝莲总黄酮对肝纤维化大鼠血清白介素-10(IL-10)表达的影响,探讨半枝莲抗大鼠肝纤维化的作用机制。方法:采用CCl4复合因素大鼠肝纤维化模型造模方法制作大鼠肝纤维化模型,采用放免法检测分析半枝莲对肝纤维化大鼠血清中丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)的含量,并通过采用双抗体夹心ELLSA法检测分析血清白介素-10(IL-10)的含量及不同剂量与疗效的关系,进一步探讨半枝莲总黄酮抗肝纤维化的可能机制。结果:半枝莲能抑制大鼠血清中ALT、AST的含量;肝纤维化评分明显降低。模型组大鼠血清中IL-10含量较其它给药组含量明显降低,各治疗组IL-10含量升高明显,与模型组比较均有显著性差异。结论:半枝莲总黄酮可升高大鼠肝纤维化模型中IL-10的表达而抑制炎症反应,减轻肝损伤,这可能是其抗肝纤维化的免疫学机制之一。
Objective: To observe the effect of Scutellaria barbata flavonoids on the expression of interleukin-10 (IL-10) in rats with hepatic fibrosis and to explore the mechanism of Scutellaria barbata against liver fibrosis in rats. Methods: Rat model of hepatic fibrosis was induced by CCl 4 -induced rat hepatic fibrosis model. Radiotherapy was used to detect the effects of Scutellaria barbata on serum alanine aminotransferase (ALT) and aspartate (AST), and to explore the relationship between serum interleukin-10 (IL-10) and different dosages and curative effect by double-antibody sandwich ELLSA to further explore the anti-hepatic fibrosis of Scutellaria barbata mechanism. Results: Scutellaria barbata could inhibit the levels of ALT and AST in serum of rats, and significantly lower the score of liver fibrosis. The content of IL-10 in the serum of the model group was significantly lower than that of the other groups, and the content of IL-10 in each treatment group increased significantly. Compared with the model group, there was a significant difference. Conclusion: Scutellaria barbata flavonoids can increase the expression of IL-10 in rat hepatic fibrosis model to inhibit the inflammatory reaction and reduce liver injury, which may be one of the immunological mechanisms of anti-liver fibrosis.