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Crohn病(CD)的病因尚不清楚。有一种假说强调组织损伤的作用,认为粘膜的完整性是肠道健康的首要因素,粘膜通透性增加引起炎症。但粘膜通透性增加的机理尚不清楚。作者发现,溶血卵磷脂可使小鼠回肠末端粘膜屏障受到损害,并提出人回肠远段刷状缘的溶血磷脂酶能防止溶血卵磷脂积聚而引起某种生理作用。推测小肠疾病时溶血磷脂酶可能像其它刷状缘酶一样缺乏或不足,而磷脂酶A_2使卵磷脂转变为溶血卵磷脂。为此,作者检测CD和非炎症性肠病(NIBD)病人回肠末端粘膜的溶血磷脂酶和磷脂酶A2活性。 CD和NIBD手术病人各6例,取肠切除标本2×5cm~2。用150mM的冰盐水冲洗干净后,取NIBD标本的粘膜上皮和CD标本肉眼正常及受累肠段的粘膜
The etiology of Crohn’s disease (CD) is unclear. There is a hypothesis that emphasizes the role of tissue damage. It is thought that the integrity of the mucosa is the primary cause of intestinal health. Inflammation is caused by increased mucosal permeability. However, the mechanism of increased mucosal permeability is not clear. The authors found that lysophosphatidylcholine can damage the terminal ileum mucosal barrier in mice, suggesting that lyso-phospholipase at the brush border of the distal ileum prevents the accumulation of lysophosphatidylcholine and causes some physiological effect. Lysosomal phospholipases may be as deficient or deficient as other brush border enzymes when speculating on intestinal diseases, whereas phospholipase A_2 converts lecithin to lysolecithin. To do this, the authors tested the activity of lysophospholipase and phospholipase A2 in the terminal ileum mucosa of patients with CD and non-inflammatory bowel disease (NIBD). CD and NIBD surgery patients in 6 cases, take intestinal resection specimens 2 × 5cm ~ 2. Rinse with 150mM ice water, take the NIBD specimens mucosal epithelium and CD specimens of normal and affected intestinal mucosa