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目的观察间充质干细胞(mesenchymal stem cells,MSCs)及聚肌苷酸胞苷酸poly(I:C)预刺激MSCs对脓毒症大鼠肠屏障功能的保护作用。方法采用盲肠结扎穿孔(cecal ligation puncture,CLP)诱导大鼠脓毒症模型,将SD大鼠随机分为5个组:正常对照组、脓毒症组、常规治疗组(乳酸林格液体复苏+血管活性药多巴胺+头孢呋辛钠)、MSCs治疗组(MSCs 3×106/只+常规治疗)和ploy(I:C)预刺激MSCs组[poly(I:C)20μg/m L预刺激MSCs 24 h+常规治疗],观察不同处理组大鼠肠通透性,血中D乳酸、细菌内毒素浓度、肠病理组织形态及动物存活时间、存活率的变化。结果与正常对照组相比,脓毒症大鼠肠通透性、血中D乳酸、细菌内毒素浓度显著增高(P<0.01),病理结果显示肠绒毛断裂、减少,上皮细胞坏死脱落,炎细胞、红细胞增多。常规治疗后第3天,同脓毒症组相比,大鼠肠通透性有改善,血D乳酸、细菌内毒素含量降低,肠绒毛损害及炎症减轻,长度增加;MSCs+常规治疗后效果明显优于常规治疗组,而poly(I:C)预刺激MSC可增强MSCs对肠屏障功能的保护作用,肠通透性及血中D乳酸、内毒素浓度显著降低(P<0.05),病理结果显示肠上皮增生修复,肠绒毛增多,炎症明显减轻。结论 poly(I:C)可增强MSC对脓毒症大鼠肠屏障功能的保护作用。
Objective To observe the protective effects of mesenchymal stem cells (MSCs) and poly (glycerophospholipid) poly (I: C) pre-stimulated MSCs on gut barrier function in septic rats. Methods The rat model of sepsis was induced by cecal ligation puncture (CLP). The SD rats were randomly divided into five groups: normal control group, sepsis group and conventional treatment group (lactic acid Ringer liquid resuscitation + MSCs treated group (MSCs 3 × 106 / only + routine treatment) and ploy (I: C) pre-stimulated MSCs group [poly (I: C) 20μg / m L preconditioning MSCs 24 h + conventional treatment]. The changes of intestinal permeability, blood lactic acid, bacterial endotoxin, intestinal histomorphology, animal survival time and survival rate in different treatment groups were observed. Results Compared with the normal control group, intestinal permeability, blood lactate and bacterial endotoxin levels in septic rats were significantly increased (P <0.01). The pathological findings showed that the villus ruptured and decreased, the necrotic epithelium necrosis and inflammation Cells, red blood cells increased. On the 3rd day after routine treatment, intestinal permeability of rats was improved, blood lactate and bacterial endotoxin levels were decreased, intestinal villi damage and inflammation were relieved, and the length was longer than that of the sepsis group. The effect of MSCs + conventional treatment was obvious Compared with the conventional treatment group, poly (I: C) pre-stimulated MSCs could enhance the protective effect of MSCs on gut barrier function, intestinal permeability and D-lactate and endotoxin concentrations in blood significantly decreased (P <0.05) Show intestinal epithelial hyperplasia repair, increased intestinal villi, inflammation was significantly reduced. Conclusion Poly (I: C) can enhance the protective effect of MSC on intestinal barrier function in septic rats.