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利用荧光指示剂Fura2测定离体青蛙半腱肌胞浆自由钙离子浓度（[Ca2+]i），已发现肌肉经长时间电刺激至力竭后，[Ca2+]i上升。对针刺影响力竭肌[Ca2+]i的机制进行了进一步探讨。研究发现，针刺正常肌使[Ca2+]i上升，其上升可被钙通道阻滞剂异博定大部分阻断；针刺电刺激后半腱肌则使[Ca2+]i下降，其下降可被Na－Ca交换阻断剂奎尼丁阻断。如在针刺前予先加入奎尼丁，可抑制针刺对电刺激肌的降钙作用。上述结果证实：1）针刺正常肌的升钙作用主要由于细胞膜慢钙通道开放：2）针刺电刺激肌的降钙作用则是通过提高细胞膜对Na+通透性，促进Na－Ca交换而完成。研究亦发现，在针刺入肌肉的留针期内，细胞内Ca2+有上升和下降两类反应。其意义与机制尚待探讨。
The free Ca 2+ concentration ([Ca 2+] i) was measured by fluorescent indicator Fura 2 in the semitendinosus of isolated frogs. It was found that [Ca 2+] i increased after prolonged electrical stimulation of the muscle to exhaustion. The mechanism of acupuncture affecting [Ca2 +] i was further explored. The study found that acupuncture normal muscle to [Ca2] i increased, the increase can be blocked by the calcium channel blocker ibuprofen mostly blocked; acupuncture electrical stimulation of the semitendinosus [Ca2] i decreased, the decline can be Interrupted by Na-Ca blocker quinidine. Such as prior to the addition of quinidine before acupuncture, can inhibit acupuncture on the electrical stimulation of calcium-lowering effect. The above results confirmed that: 1) The acupuncture of normal muscle is mainly due to the opening of the slow calcium channel in the cell membrane; 2) The acupuncture stimulates the calcium-lowering effect of the muscle by increasing the membrane permeability to Na + and promoting Na-Ca exchange carry out. The study also found that in the acupuncture needle into the muscle during the intracellular Ca2 + rise and fall two types of reactions. Its significance and mechanism remains to be explored.