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[目的]寻找理想的锰中毒解毒剂。[方法]小鼠一次性腹腔注射MnCl(2100mg MnCl2/kg)24h后分别给予L-组氨酸(L-His)、乙二胺四乙酸(EDTA)、二乙基二硫代氨基甲酸钠(DDTC)、meso-2,3-二巯基丁二酸(DMSA)及N-乙酰-L-半胱氨酸(NAC)等五种螯合剂(2mmol/kg,腹腔注射),观察小鼠肺脏、睾丸脂质过氧化物值(LPO)和组织中锰、钙、锌浓度的变化。[结果]小鼠一次性腹腔注射MnCl(2100 mg MnCl2/kg)2d后,小鼠肺脏和睾丸中LPO值分别为(2.42±0.29)和(2.74±0.33)μmol/g.prot,锰浓度分别为(2.60±0.33)和(2.41±0.68)μg/g,均明显高于对照组,差异有统计学意义(P<0.05)。分别染锰24h后腹腔注射各螯合剂,治疗24h后L-His对锰引起的肺脏、睾丸LPO值和锰浓度的升高有显著的抑制作用。[结论]L-His对锰致小鼠肺脏和睾丸毒性有较好的解毒作用,且自身毒性小,有望成为理想的锰解毒剂。
[Objective] To find the ideal manganese poisoning antidote. [Method] Mice were given intraperitoneal injection of MnCl (2100 mg MnCl2 / kg) for 24 h and then treated with L-His, EDTA, DDTC ), Meso-2,3-dimercaptosuccinic acid (DMSA) and N-acetyl-L-cysteine (NAC) Lipid peroxidation value (LPO) and changes in tissue manganese, calcium, zinc concentration. [Results] The LPO values of lung and testis in mice were (2.42 ± 0.29) and (2.74 ± 0.33) μmol / g.prot after a single intraperitoneal injection of MnCl (2100 mg MnCl2 / kg) (2.60 ± 0.33) and (2.41 ± 0.68) μg / g, respectively, which were significantly higher than those in the control group (P <0.05). After 24 hours of manganese exposure, the chelators were injected intraperitoneally. After treatment for 24 hours, L-His significantly inhibited the increase of manganese and LPO levels in the lung and testis. [Conclusion] L-His has good detoxification effect on lung and testicular toxicity induced by manganese, and its own toxicity is small, which is expected to become an ideal manganese antidote.