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目的观察摘除颈上神经节(SCG)对成年大鼠海马基质金属蛋白酶9(MMP9)和水通道蛋白9(AQP9)表达的影响,探索交感神经对脑组织的调节作用及机制。方法 40只成年健康雄性Wistar大鼠分为对照组和手术组,手术组大鼠摘除双侧SCG;用免疫组织化学法对海马区MMP9及AQP9蛋白的表达进行定位,Western blotting技术对海马区MMP9及AQP9蛋白的表达进行定量分析,RT-PCR技术测定海马区MMP9 mRNA及AQP9mRNA的表达变化。结果 MMP9免疫阳性反应产物表达于神经元和神经胶质细胞,手术组海马区MMP9的表达明显强于对照组,CA1区和CA3区的阳性神经元在锥体细胞层散在分布,齿状回(DG)区的强阳性细胞主要位于颗粒细胞层;手术组海马MMP9蛋白表达和MMP9 mRNA表达水平明显升高,和对照组相比差异极显著(P<0.01)。AQP9免疫阳性反应产物在神经元、神经胶质细胞和血管均有表达,手术组AQP9的表达明显强于对照组,DG区的强阳性细胞主要位于颗粒细胞下层的新生细胞,且室管膜细胞中也有AQP9的高表达;手术组AQP9蛋白表达和AQP9 mRNA表达水平均明显增强,与对照组相比差异极显著(P<0.01)。结论摘除成年大鼠SCG 15d后,海马MMP9、AQP9的表达明显上调,表明脑部去交感所引起的脑供血的改变会对海马多个因子的表达产生影响而发挥调节作用。
Objective To observe the effect of removing upper cervical ganglion (SCG) on the expression of matrix metalloproteinase 9 (MMP9) and aquaporin 9 (AQP9) in hippocampus of adult rats and explore the regulatory mechanism of sympathetic nerve on brain tissue. Methods Forty adult healthy male Wistar rats were divided into control group and operation group. The rats in operation group were enrolled in the study. The expression of MMP9 and AQP9 protein in hippocampus was detected by immunohistochemistry. The expression of MMP9 And AQP9 protein expression quantitative analysis, RT-PCR technology determination of hippocampus MMP9 mRNA and AQP9 mRNA expression changes. Results The expression of MMP9 was positive in neurons and glial cells. The expression of MMP9 in the hippocampus of the operation group was significantly stronger than that in the control group. The positive neurons in CA1 and CA3 were scattered in the pyramidal cell layer, DG) were mainly located in the granular cell layer. The expression of MMP9 and MMP9 mRNA in the hippocampus of the operation group was significantly higher than that of the control group (P <0.01). The positive expression of AQP9 in neurons, glial cells and blood vessels was observed. The expression of AQP9 in the operation group was significantly stronger than that in the control group. The strong positive cells in DG region were mainly located in the neutrophils of granulosa cells and the ependymal cells AQP9 protein expression and AQP9 mRNA expression levels were significantly increased in the operation group, compared with the control group, the difference was significant (P <0.01). Conclusion The expression of MMP9 and AQP9 in the hippocampus was significantly upregulated after the SCG was removed in adult rats 15 days, indicating that the alteration of cerebral blood supply induced by deacetylation of the brain may play an important role in the regulation of multiple factors in the hippocampus.