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从Vero细胞培养液中提纯培养的汉滩病毒 ,将细胞冻融后上清液过Sepharose 4FF凝胶层析柱 ,经紫外线2 80nm波长检测到三个吸收峰 ,RPHA证实仅第一峰为病毒抗原峰。另二个峰为杂蛋白。实验说明Sepharose4FF凝胶过滤对于提纯HFRS汉滩病毒是非常有效的 ,能去除 98%的杂蛋白
The Hantaan virus was purified from Vero cell culture medium, and the supernatant was frozen and thawed. The supernatant was passed through Sepharose 4FF gel column and detected by ultraviolet at 280 nm. RPHA confirmed that only the first peak was virus Antigen peak. The other two peaks for the hybrid protein. Experimental Description Sepharose 4FF gel filtration is very effective for the purification of HFRS Hantaan virus, which can remove 98% of the hybrid protein