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目的:为了建立一种组织块培养雪旺细胞的有效方法,为周围神经组织工程修复的研究提供种子细胞。方法:本研究从新生大鼠的周围神经分离雪旺细胞,经组织块法培养并纯化,观察并记录其形态特征;经HE染色以及S-100免疫组化染色鉴定并计算其纯度。结果:结果显示,4 d后,获得的雪旺细胞突起呈双极或三极,S-100阳性细胞纯度达到95%以上。结论:上述结果表明该方法快速有效,所获得的雪旺细胞纯度高、活性好。
OBJECTIVE: To establish an effective method for culturing Schwann cells in tissue blocks and to provide seed cells for the study of tissue engineering repair of peripheral nerves. Methods: Schwann cells were isolated from the peripheral nerve of neonatal rats, cultured and purified by tissue block method. Morphological characteristics were observed and recorded. The purity of Schwann cells was identified by HE staining and S-100 immunohistochemistry. Results: The results showed that after 4 days, Schwann cells were bipolar or tripolar, and the purity of S-100 positive cells was over 95%. Conclusion: The above results show that the method is rapid and effective, and the obtained Schwann cells have high purity and good activity.