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目的:通过HPLC测定黄芩和半夏不同配伍比例后黄芩苷溶出量的变化,以探讨中药药性配伍的科学内涵。方法:采用Diamonsil C18(2)柱(4.6 mm×250 mm,5μm),乙腈-0.4%磷酸溶液(26∶74)为流动相,检测波长280 nm,流速1.0 mL.min-1,柱温30℃。建立黄芩与半夏配伍[以寒为主(9∶6);寒热各半(9∶9);以热为主(6∶9)]后黄芩苷的含量测定方法,并比较不同配伍后黄芩苷溶出量的大小。结果:黄芩苷质量浓度在0.010 25～0.102 5 g.L-1线性关系良好(r=0.999 9),平均回收率99.21%(n=9),RSD 2.0%;黄芩配伍半夏后[以寒为主(9∶6)、寒热各半(9∶9)、以热为主(6∶9)]和黄芩单煎后黄芩苷平均含量分别为64.018,63.6805,0.581,62.529 mg.g-1。结论:黄芩配伍半夏后黄芩苷的HPLC检测方法可靠、稳定、重复性好。不同比例配伍后黄芩苷的溶出量有较大差异,尤其是以热为主配伍(6∶9)对黄芩苷的溶出影响最大,溶出最低,体现了中药药性配伍中以半夏的温热之性抑制黄芩之寒凉之性的科学内涵。
OBJECTIVE: To determine the change of baicalin dissolution after different compatibility of Radix Scutellariae and Pinellia by HPLC to explore the scientific connotation of compatibility of traditional Chinese medicine. METHODS: Diamonsil C18 column (4.6 mm × 250 mm, 5 μm) and acetonitrile-0.4% phosphoric acid solution (26:74) were used as the mobile phase. The detection wavelength was 280 nm and the flow rate was 1.0 mL · min- ℃. Establish baicalin and Pinellia compatibility [mainly cold (9: 6); cold and a half (9: 9); mainly to heat (6: 9)] baicalin content determination method, and compare different compatibility Scutellaria Glycosidation of the size of the amount. Results: The linearity of baicalin was good (r = 0.999 9) with the average recovery of 99.21% (n = 9) and 2.0% of RSD in the range of 0.010 25-0.102 5 gL-1. (9:6), cold and heat half (9: 9), the main heat (6: 9)] and baicalin per baicalin average content were 64.018,63.6805,0.581,62.529mg.g-1. Conclusion: The HPLC method of baicalin combined with Radix Scutellariae is reliable, stable and reproducible. Different proportions of baicalin dissolution are quite different, especially the heat-based compatibility (6: 9) on the dissolution of baicalin greatest impact, the lowest dissolution, reflects the compatibility of traditional Chinese medicine in Pinellia warm Scientific Connotation of Inhibiting the Cold and Cold Nature of Scutellaria.