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目的:检测免疫性肝损伤小鼠的阴虚证表征,观察一贯煎对阴虚证表征的影响。方法:将80只雄性BALB/c小鼠随机分为正常对照组、模型组、阳性对照组、一贯煎组,每组20只。除正常对照组外,均建立免疫性肝损伤小鼠模型,在造模同时,一贯煎组小鼠予一贯煎浓缩液灌胃,0.4 m L/20g,1次/d,连续8周。阳性对照组给予联苯双酯灌胃,0.2 g/kg,1次/d,连续8周。正常对照组和模型组按相同方法灌服等量生理盐水。8周后,检测各组小鼠血清ALT、AST等肝功能指标;通过HE染色评价肝脏炎症活动度(G)和纤维化程度(S);检测小鼠体质量、舌面干湿度、舌温、舌和肝脏微循环血流速度、食物和饮水、大小便等阴虚证表征。结果:(1)一贯煎在改善肝功能、减轻炎症、抗肝纤维化方面作用较好(P<0.05)。(2)免疫性肝损伤小鼠具有阴虚证的表征,一贯煎能够改善阴虚证的症状(P<0.05)。结论:免疫性肝损伤小鼠具有阴虚证的特点;一贯煎能改善肝损伤小鼠的病理及阴虚证的症状。
Objective: To detect Yin deficiency syndrome in immunological liver injury mice and to observe the effect of Jian-Jian on Yin deficiency syndrome. Methods: Eighty male BALB / c mice were randomly divided into normal control group, model group, positive control group and consistent fried group, with 20 rats in each group. In addition to the normal control group, mice model of immune liver injury were established. At the same time of modeling, the mice in the same experimental group were treated with orally administered concentrated concentrate, 0.4 m L / 20g, once daily for 8 weeks. The positive control group was given bifendate gavage, 0.2 g / kg, 1 / d for 8 weeks. The normal control group and model group were fed the same amount of saline by the same method. After 8 weeks, the serum ALT, AST and other liver function indexes of mice in each group were detected. The hepatic inflammatory activity (G) and fibrosis degree (S) were evaluated by HE staining. The body weight, , Tongue and liver microcirculation blood flow velocity, food and water, urine and other Yin deficiency syndrome. Results: (1) Consistently improved the liver function, reduce inflammation, anti-liver fibrosis better (P <0.05). (2) Mice with immunological liver injury have the characteristics of yin deficiency syndrome, which has been shown to improve the symptoms of yin deficiency syndrome (P <0.05). Conclusion: Immunological liver injury in mice with yin deficiency syndrome characteristics; consistent Jian can improve liver injury in mice pathology and yin deficiency syndrome.