自1983年Simoni等建立孕早期绒毛细胞直接制备染色体的方法后,又出现了短期培养法。国内外学者不断努力使制片效果显著提高,并已应用于临床诊断。但是,迄今仅 FinnSondergaard 等报告用培养法制备孕早期绒毛细胞的高分辨染色体(荧光 R 带)。因 R 带不如 G 带 Since 1983, Simoni et al. Established a method of directly preparing chromosomes in the first trimester villi cells and then short-term culture method appeared. Domestic and foreign scholars continue to strive to make the film significantly improved, and has been used in clinical diagnosis. However, to date, only FinnSondergaard et al. Report the use of culture to prepare high resolution chromosomes (fluorescent R bands) of early-stage villi cells. Because the R band is inferior to the G band