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目的观察昆明(Kunming,KM)小鼠输卵管中氧化应激和抗氧化基因的表达是否随动情周期而改变。方法分别取动情期和动情间期小鼠输卵管进行RNA提取,用PCR芯片检测氧化应激和抗氧化基因表达的差别,然后用Real Time PCR对差异表达基因进一步验证。结果与动情间期比较,动情期小鼠输卵管中NADPH氧化酶1(NADPH oxidase1,NOX1)、谷胱甘肽过氧化物酶2(glutathion peroxidase 2,GPX2)、超氧化物歧化酶3(superoxide dismutase 3,SOD3)、一氧化氮合酶2(nitric oxide synthase 2,NOS2)等4个基因表达上调。结论动情期小鼠输卵管中NOX1、GPX2、SOD3和NOS2等4个基因表达上调,提示其可能参与维持动情期小鼠输卵管腔内适度的活性氧自由基(reactive oxygen species,ROS)和一氧化氮(nitric oxide,NO)水平,为卵子受精和早胚发育提供必要第二信号分子。
Objective To observe whether the expression of oxidative stress and antioxidant genes in fallopian tubes of mice in Kunming (KM) mice changed with estrous cycle. METHODS RNA was extracted from the fallopian tubes of estrus and estrus during ovulation. The differences between oxidative stress and antioxidant genes were detected by PCR. Then the differentially expressed genes were further verified by Real Time PCR. Results Compared with estrous interval, the expression of NADPH oxidase 1 (NOX1), glutathion peroxidase 2 (GPX2), superoxide dismutase 3 3, SOD3) and nitric oxide synthase 2 (NOS2) were up-regulated. Conclusions Overexpression of NOX1, GPX2, SOD3 and NOS2 in the oviduct of estrus mice was up-regulated, suggesting that they may be involved in maintaining proper ovarian reactive oxygen species (ROS) and nitric oxide (nitric oxide, NO) level, providing the necessary second signal molecules for fertilization and early embryo development.