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目的:用逆行标记方法观察脑干神经元向皮质扣带回中部(the middle portion of the cingulate cortex,MCC)的纤维投射。方法:立体定位注射法将0.06~0.08μl的4%荧光金(Fluoro-gold,FG)注射至MCC(Bregmma:+0.2,-0.3,-0.8 mm)部位,7 d后处死大鼠,含4%多聚甲醛的磷酸缓冲液(PB)灌注固定,取脑组织,制作30μm厚度的冰冻切片,荧光显微镜下观察FG逆行标记神经元的定位分布并计数。结果:MCC与皮质间有广泛的联系,包括与同侧、对侧和扣带回内部的联系。在脑干中缝核簇、蓝斑、被盖腹侧区、被盖背内侧区及中脑中央灰质(α部)均可观察到FG逆标神经元的分布,以上FG标记神经元以同侧为主,对侧较少。结论:以上核团向MCC的投射表明MCC接受来自脑干神经元的广泛投射,提示MCC神经元的活动受到脑干众多结构的调控。
OBJECTIVE: To observe the projection of brainstem neurons to the middle portion of the cingulate cortex (MCC) by retrograde labeling. METHODS: Fluoro-gold (FG) 0.06 ~ 0.08μl was injected into the MCC (Bregmma: + 0.2, -0.3, -0.8mm) site by stereotaxic injection. % Paraformaldehyde phosphate buffer (PB) perfusion fixation, brain tissue was taken, the thickness of 30μm frozen sections were made, observed under fluorescence microscope retrograde labeling neurons localization and counting. RESULTS: There was extensive association between the MCC and the cortex, including the internal connections with the ipsilateral, contralateral and cingulate. The distributions of FG retrograde neurons were observed in the nucleus raphe cluster, the locus coeruleus, the ventral tegmental area, the dorsomedial medial area and the central gray matter (α portion) of the brain stem. The above FG-labeled neurons were located on the same side Mainly less contralateral. CONCLUSIONS: The projection of the above nucleus to the MCC suggests that MCCs receive extensive projection from brainstem neurons, suggesting that the activities of MCC neurons are regulated by numerous structures in the brainstem.