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建立了检测HHV-6病毒特异DNA的PCR方法,对43份健康献血员外周血淋巴细胞DNA进行了检测,HHV-6病毒DNA的阳性率为18.6%。同时从HHV-6中国分离株CH3中克隆到其主要衣壳蛋白(MCP)基因片段,并进行了序列分析和同源性比较。
A PCR method for detecting specific DNA of HHV-6 virus was established. The DNA of 43 peripheral blood lymphocytes from healthy blood donors was tested. The positive rate of HHV-6 DNA was 18.6%. At the same time, the major capsid protein (MCP) gene fragment was cloned from CH3 of HHV-6, and the sequence analysis and homology comparison were carried out.