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目的探讨白藜芦醇(Resveratrol,Res)对大鼠脑缺血再灌注氧化应激损伤的影响。方法将SD大鼠随机分为假手术组(S组)、缺血再灌注组(I/R组,线栓法复制大鼠右侧大脑中动脉栓塞模型)、Res低剂量组(15 mg/kg,I/R+R1组)和Res高剂量组(30 mg/kg,I/R+R2组),于缺血2 h再灌注24 h进行神经功能缺损评分;化学比色法测定大鼠血清和脑组织中丙二醛(Malondialdehyde,MDA)含量及超氧化物歧化酶(Superoxide dismutase,SOD)活性;TTC法测定脑梗死体积;干湿重法测定脑含水量,HE染色观察脑组织的病理改变。结果与I/R组相比,Res能改善大鼠脑缺血再灌注损伤后的神经功能缺失(P<0.01),降低血清及脑组织中MDA的含量(P<0.01),提高SOD活性(P<0.01),缩小脑梗死体积(P<0.01),降低损伤侧脑含水量(P<0.01),改善脑组织的病理变化,且呈剂量依赖性。结论 Res对大鼠局灶性脑缺血再灌注氧化应激损伤具有良好的保护作用,其机制可能与清除自由基,减轻氧化性损伤有关。
Objective To investigate the effect of resveratrol (Res) on oxidative stress injury induced by cerebral ischemia-reperfusion in rats. Methods SD rats were randomly divided into three groups: sham operation group (S group), ischemia reperfusion group (I / R group, middle cerebral artery occlusion model by thread method), Res low dose group (15 mg / kg and I / R + R1 group) and Res high dose group (30 mg / kg and I / R + R2 group) for neurological deficit score 24 h after ischemia. Serum and brain tissue contents of malondialdehyde (MDA) and superoxide dismutase (SOD) were measured. The volume of cerebral infarction was measured by TTC method. The brain water content was measured by wet and dry method. Pathological changes. Results Compared with I / R group, Res improved neurological deficits (P <0.01), decreased MDA content in serum and brain tissue (P <0.01) and increased SOD activity (P < P <0.01). The volume of cerebral infarction was decreased (P <0.01), the content of water in the injured side of brain was decreased (P <0.01), and the pathological changes of brain tissue were improved in a dose-dependent manner. Conclusion Res has a good protective effect on oxidative stress induced by focal cerebral ischemia / reperfusion in rats. The mechanism may be related to scavenging free radicals and reducing oxidative damage.