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目的应用生物信息学方法分析广州地区人巨细胞病毒(human cytomegalovirus,HCMV)临床低传代病毒株D3的UL147基因B细胞抗原表位。方法应用生物信息学方法:用TMHMM预测跨膜区域,用改进型自我优化预测结构(SOPMA)、Garnier-Osguthorpe-Robson(GOR)法、人工神经网络预测法(HNN)预测二级结构,用Hopp&Woods亲水性方案、Janin可及性方案、Welling抗原性方案等参数预测UL147基因抗原表位。结果临床低传代HCMV病毒株D3的UL147基因包含159个氨基酸残基,等电点为9.56;UL147存在一个跨膜区域,为20~42aa区域,胞外区域为1~19aa区域;无规则卷及β-转角区域主要位于12~17、22~28、31~48、99~109、130~145aa区域;亲水性位于15~39、101~111、135~143aa区域;可及性位于14~45、103~124、131~143aa区域;抗原性位于9~14、63~70、108~116aa区域。结论临床低传代HCMV病毒株UL147基因抗原表位可能位于7~12aa区域或其附近。
OBJECTIVE: To analyze the B cell epitopes of UL147 gene in Guangzhou low-generation human cytomegalovirus (HCMV) clinical low-pass strain D3 by bioinformatics method. Methods The bioinformatics method was used to predict the transmembrane region by using TMHMM. The secondary structure was predicted by improved self-optimizing predicted structure (SOPMA), Garnier-Osguthorpe-Robson (GOR) and artificial neural network prediction (HNN) Hydrophilicity regimen, Janin accessibility regimen, Welling antigenic regimen and other parameters to predict UL147 antigenic epitopes. Results UL147 gene of clinical low passage HCMV strain D3 contained 159 amino acid residues and had an isoelectric point of 9.56. UL147 had a transmembrane region of 20-42aa and an extracellular region of 1-9aa. The β-turn region is mainly located in the region of 12 ~ 17,22 ~ 28,31 ~ 48,99 ~ 109,130 ~ 145aa; the hydrophilicity is in the region of 15 ~ 39,101 ~ 111,135 ~ 143aa; 45,103 ~ 124,131 ~ 143aa region; antigenicity located in 9 ~ 14,63 ~ 70,108 ~ 116aa region. Conclusion The clinical manifestation of UL147 gene epitope of low passage HCMV strain may be located in or near the region of 7 ~ 12aa.