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１７α－羟化酶催化雄激素合成的中间步骤，它对了解睾丸间质细胞内雄激素的合成具有重要的意义。本研究首先提取小鼠睾丸组织ＲＮＡ，然后以１７α－轻化酶ＤＮＡ两端的特定序列为引物，反转录合成ｃＤＮＡ，再以ｃＤＮＡ为模板行ＰＣＲ扩增ｃＤＮＡ。结果显示，反转录ＰＣＲ后获得一条３６０ｂｐ的电泳带，为１７α－羟化酶ｃＤＮＡ。结果表明，用反转录ＰＣＲ方法扩增睾丸１７α－羟化酶ｍＲＮＡ是切实可行的。
17α-hydroxylase catalyzes the intermediate step of androgen synthesis, which is of great significance for understanding androgen synthesis in testicular stromal cells. In this study, the RNA of mouse testis tissue was first extracted, and then cDNA was reverse transcribed using a specific sequence of 17α-light enzyme DNA as a primer. Then the cDNA was amplified by PCR. The results showed that a 360bp electrophoresis band was obtained after RT-PCR and was 17α-hydroxylase cDNA. The results showed that reverse transcription PCR amplification of testicular 17α-hydroxylase mRNA is feasible.