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目的研究脂膜微囊(LCM)靶向性聚集于大鼠颅内C6胶质瘤动物模型的机制。方法建立大鼠C6胶质瘤动物模型,成瘤大鼠分为两组:单次注射LCM组,多次连续注射LCM组,处死后获取大鼠脑组织标本,进行常规HE染色、油红O染色,荧光显微镜观察LCM在肿瘤位点分布的特点,取C6胶质瘤细胞研究LCM在细胞内的分布特点。结果油红O染色显示肿瘤细胞内有LCM的聚集,且多次注射LCM组其LCM浓度明显较单次注射组高。荧光显微镜观察发现,LCM主要聚集于肿瘤区,且主要分布于肿瘤细胞的胞浆中。对体外培养的C6胶质瘤细胞研究发现,LCM先结合于细胞表面,随后在细胞内再分布,其在细胞内的分布可能与细胞内的酸性成分有关。结论LCM可靶向性聚集于肿瘤位点,其在肿瘤位点浓度与注射次数成正相关。其靶向性机制与LCM的脂质构成有关,还与脑肿瘤引起的局部血脑屏障破坏有关。LCM在肿瘤细胞内的代谢与细胞内的酸性成分有关。
Objective To study the mechanism of targeted aggregation of lipid microcapsules (LCMs) in rat C6 glioma model. Methods The animal model of C6 glioma was established. Tumor-bearing rats were divided into two groups: single injection of LCM group, continuous injection of LCM group, brain tissue samples of rats after sacrifice, routine HE staining, oil red O Staining, fluorescence microscopy LCM distribution in the tumor site characteristics, take C6 glioma cells to study the distribution of LCM in the cell characteristics. Results Oil red O staining showed the accumulation of LCM in tumor cells, and LCM concentration in multiple injection LCM group was significantly higher than that in single injection group. Fluorescence microscopy found that LCM mainly gathered in the tumor area, and mainly distributed in the cytoplasm of tumor cells. In vitro cultured C6 glioma cells found that LCM first bound to the cell surface, and then redistributed within the cell, its intracellular distribution may be related to intracellular acidic components. Conclusion LCM can be targeted at the tumor site, and its concentration at the tumor site is positively correlated with the number of injections. Its targeting mechanism is related to the lipid composition of LCM, but also to the destruction of the local blood-brain barrier caused by brain tumors. The metabolism of LCM in tumor cells is related to the intracellular acidic components.