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由于电子设备的广泛使用,人类已经越来越多地暴露在射频磁场的辐射下,但射频磁场的辐射效应却一直不明确.采用全细胞膜片钳技术,记录2 450 MHz射频磁场辐射对小鼠脑皮层神经元延迟整流钾电流IK的影响.利用Ansoft HFSS软件对6 dB全向天线建模仿真,验证距天线2～3 cm处磁场分布均匀,使用Agilent E5070B网络分析仪经该天线发射出2 450 MHz输出功率为39.81mW电磁场,对细胞进行刺激.实验发现,2 450 MHz低功率射频磁场暴露5、10和15 min对IK均有明显的抑制作用;显著影响IK激活特性,对照组与磁场暴露组半数激活电压分别为(-1.13±2.32)mV和(19.52±1.03)mV(n=10,P<0.05);斜率因子分别为(23.21±3.29)mV和(13.95±1.27)mV(n=10,P<0.05).结果表明,低功率射频磁场通过减小延迟整流钾通道电流,影响神经元的生理功能,为进一步研究电磁辐射所引发的生物学效应提供了一种新的方法.
Due to the widespread use of electronic devices, human beings have been increasingly exposed to radio frequency magnetic field radiation, but the radio frequency magnetic field radiation effects have not been clear.Using whole cell patch clamp technology, recording 2 450 MHz RF magnetic field radiation on mice Cortical neurons delayed rectifier potassium current IK.Using Ansoft HFSS software modeling and simulation of 6 dB omnidirectional antenna to verify the magnetic field distribution 2 ~ 3 cm away from the antenna evenly distributed using the Agilent E5070B network analyzer 2 450 MHz output power of 39.81mW electromagnetic field stimulation of cells.It was found that 2 450 MHz low-power RF magnetic field exposed to 5,10 and 15 min on IK were significantly inhibited; significantly affect IK activation characteristics of the control group and the magnetic field The half activation voltages of the exposed group were (-1.13 ± 2.32) mV and (19.52 ± 1.03) mV (n = 10, P <0.05), respectively; the slope factors were (23.21 ± 3.29) mV and (13.95 ± 1.27) mV = 10, P <0.05). The results show that the low power RF magnetic field can reduce the delayed rectifier potassium channel current and affect the neuronal physiological function, which provides a new method for further studying the biological effects caused by electromagnetic radiation.