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目的:研究基因MMP-9-1562位点基因多态性与肠道病毒71型(EV71)脑炎的关系,探讨不同基因型对EV71脑炎患病风险及病情程度的影响。方法:运用限制性片段长度多态性-聚合酶链反应(PCR-RFLP)检测EV71感染阳性病毒性脑炎患儿及正常对照组儿童基因组MMP-9-1562位点碱基变异情况。结果:EV71脑炎重症组MMP-9-1562位点T等位基因频率分布明显高于EV71脑炎轻症组(16%VS 7.5%,OR 2.362,95%CI 1.170~4.768,P<0.05)。MMP-9-1562位点T等位基因携带者感染EV71后可明显增加神经系统并发症的发生率。EV71脑炎患儿MMP-9-1562位点T等位基因频率分布与正常对照儿童比较无明显差异。结论:MMP-9-1562位点T等位基因携带者在感染EV71后易发展为重症中枢神经系统感染;MMP-9-1562位点T等位基因可增加EV71感染后神经系统并发症的发生率;MMP-9-1562位点单核苷酸多态性与EV71脑炎的发病率无关。
OBJECTIVE: To study the relationship between gene polymorphism of MMP-9-1562 locus and enterovirus 71 (EV71) encephalitis and explore the influence of different genotypes on the risk and severity of EV71 encephalitis. Methods: The genomic polymorphisms of MMP-9-1562 in children with EV71-positive viral encephalitis and controls were detected by restriction fragment length polymorphism-polymerase chain reaction (PCR-RFLP). Results: The frequencies of T allele in EV71 encephalitis severe group were significantly higher than those in EV71 encephalitis mild group (16% vs 7.5%, OR 2.362, 95% CI 1.170 ~ 4.768, P <0.05) . The infection of EV71 with T-allele of MMP-9-1562 could significantly increase the incidence of neurological complications. The frequencies of T allele in MMP-9-1562 in children with EV71 encephalitis had no significant difference compared with those in normal controls. Conclusion: The carriers of T allele at MMP-9-1562 locus develop severe CNS infection after EV71 infection. The T allele at MMP-9-1562 site may increase the incidence of neurological complications after EV71 infection Rate. The single nucleotide polymorphism of MMP-9-1562 was not associated with the incidence of EV71 encephalitis.