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本文报道一种快速提纯血清α_1酸性糖蛋白抗原(α_1AGP)的新方法。从人血清中性盐(硫酸铵)50％饱和度的上清液中,用K_s分段及β分段盐析法先获得α_1AGP的粗制品,再经离子交换纤维素柱层析得到电泳纯的α_1AGP。此法简便快速,获得率高,重复性好,且在提纯过程中其它蛋白质不发生变性,可同时获得β_1球蛋白纯品,也可用此法进一步分离γ球蛋白及白蛋白。所得α_1AGP免疫动物得抗血清,经免疫扩散及免疫电泳证实为免疫纯蛋白。所得抗血清为单价抗血清,可用于临床进行免疫化学检查。
This article reports a new method for the rapid purification of serum α_1 acid glycoprotein antigen (α_1AGP). From the supernatant of human serum neutral salt (ammonium sulphate) 50% saturation, crude α_1 AGP products were first obtained by K_s segmentation and β-segmentation salting-out, and then purified by ion exchange cellulose column chromatography to obtain pure electrophoresis. Α_1 AGP. This method is simple and rapid, with high acquisition rate and good repeatability. Other proteins do not denature during the purification process. Pure β1-globulin can be obtained at the same time. This method can also be used to further separate γ-globulin and albumin. The obtained α_1 AGP immunized animals to obtain antiserum, which was confirmed by immunodiffusion and immunoelectrophoresis as an immunopure protein. The resulting antiserum is a monovalent antiserum that can be used for clinical immunochemistry.