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IL-12主要是由B 淋巴细胞分泌的异源双体细胞因子。cDNA 克隆和表达已获成功。最初发现IL-12能与亚适剂量IL-2协同诱导细胞毒性效应细胞产生,因而被命名为细胞毒性淋巴细胞成熟因子(CLMF)。随后又发现IL-12能刺激PHA 活化的90%以上CD_3~+T 淋巴母细胞增殖。本文作者主要研究了IL-12在调节静止及活化的外周血单个核细胞(PBMC)增殖中作用。常规分离PBMC,阴性选择贴附法分离T,B,NK 细胞及CD_4,CD_8亚群,免疫荧光染色和流式细胞仪分析细胞表型,~3H—TdR 掺入法检测细胞增殖水平。
IL-12 is predominantly a heterobicygenar cell factor secreted by B lymphocytes. cDNA cloning and expression have been successful. IL-12 was initially found to synergistically induce the production of cytotoxic effector cells in combination with an appropriate amount of IL-2 and was therefore named CLMF (cytotoxic lymphocyte maturation factor). Subsequently, IL-12 was also found to stimulate PHA-activated proliferation of more than 90% of CD3 + T lymphoblasts. The authors mainly studied the role of IL-12 in the regulation of the proliferation of resting and activated peripheral blood mononuclear cells (PBMCs). PBMCs were isolated routinely, T, B and NK cells, CD_4 and CD_8 subgroups were separated by negative selection, and cell phenotypes were analyzed by immunofluorescence staining and flow cytometry. Cell proliferation was measured by 3H-TdR incorporation assay.