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目的 :探讨肝细胞生长因子 (HGF)体外诱导骨髓间质干细胞 (MSC)定向分化心肌细胞的一种新方法。方法 :分离SD大鼠骨髓MSC ,培养至 4 - 6代后 ,添加HGF(终浓度 10 μg/L)持续培养 30d ,倒置显微镜下动态观察分化细胞的自律性搏动和对 0 1%异丙肾上腺素和无Ca2 + 孵育液的反应 ,并行心肌肌凝蛋白表达鉴定。结果 :分化第 14 - 2 0d骨髓间质干细胞增殖形成集落 ,10 % - 5 0 %克隆中开始出现持续节律收缩的细胞群体 ,节律频率为 70- 90次 /min ,异丙肾上腺素加快其搏动速率 ,无Ca2 + 孵育液则抑制之。细胞心肌肌凝蛋白表达鉴定为心肌细胞。结论 :HGF可诱导骨髓间质干细胞分化为心肌细胞 ,且有较高的诱导效率
AIM: To investigate a new method of inducing bone marrow mesenchymal stem cells (MSC) to differentiate into cardiomyocytes in vitro by hepatocyte growth factor (HGF). Methods: Bone marrow MSCs from SD rats were isolated and cultured. After 4-6 days of culture, HGF (final concentration 10 μg / L) was cultured continuously for 30 days. The autonomic pulsation of differentiated cells and the changes of the morphology of 0 1% Response to Ca2 + and Ca2 + incubation solutions was performed in parallel with myocardial myosin expression. RESULTS: Bone marrow mesenchymal stem cells (MSCs) proliferated to form colonies on the 14th to 20th day of differentiation, and cell populations with sustained rhythm contractions began to appear in 10% to 50% of the clones. The frequency of rhythm was 70-90 beats / min and isoproterenol accelerated its beating Rate, no Ca2 + incubation solution is inhibited. Myocardial cell myosin expression was identified as cardiomyocytes. Conclusion: HGF can induce bone marrow mesenchymal stem cells to differentiate into cardiomyocytes with higher induction efficiency