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目的:比较5种药用菊花试管苗在离体条件下对羟脯氨酸的耐受性,为药用菊花引种栽培和育种提供依据。方法:以5种药用菊花试管苗为材料,接种于添加不同浓度羟脯氨酸的培养基中培养,测定植株叶片内游离脯氨酸的含量,并统计植株的受害指数和存活率。结果和结论:在高浓度羟脯氨酸胁迫下,亳菊和怀菊试管苗受害指数较低,成活率相对较高,叶片内游离脯氨酸含量高,具有较强的HYP耐受性。滁菊和贡菊植株存活率低于亳菊和怀菊,表现为中等的HYP耐受性。杭菊试管苗存活率最低,HYP耐受性最差。
OBJECTIVE: To compare the tolerance of 5 medicinal chrysanthemum plantlets to hydroxyproline under in vitro conditions and to provide the basis for introduction, cultivation and breeding of medicinal chrysanthemum. Methods: Five kinds of medicinal chrysanthemum plantlets were inoculated into medium supplemented with different concentrations of hydroxyproline. The contents of free proline in the leaves of the plants were measured. The damage index and survival rate of the plants were calculated. RESULTS AND CONCLUSION: Under high concentration of hydroxyproline stress, the damage index and the HYP tolerance of test-tube plantlets of Boju and Daisy were lower, the survival rate was relatively higher, the free proline content in leaves was higher. The survival rate of the plants of Chrysanthemum and Gongju was lower than that of the ones of Boju and Daisy, showing moderate HYP tolerance. Hang Chrysanthemum in vitro survival rate of the lowest HYP tolerance of the worst.