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目的考察钠基蒙脱土(Na-montmorillonite,Na-MMT)对桑叶提取物中1-脱氧野尻霉素(1-deoxynojirimycin,DNJ)是否存在缓释效果。方法在搅拌条件下使其进入钠基蒙脱土层间,从而制备1-脱氧野尻霉素-蒙脱土复合物。通过计算钠基蒙脱土对1-脱氧野尻霉素的载药量来选择合适的吸附时间、温度、投料比,并对该条件下制备的1-脱氧野尻霉素-蒙脱土复合物进行体外实验,评价缓释效果。结果当钠基蒙脱土用量为5.00 g,桑叶提取物用量16.00 g(1-脱氧野尻霉素含量25.1%),在100℃下,于1 000 m L去离子水中搅拌1 h时,可制得1-脱氧野尻霉素载药量为111.64 mg·g-1的1-脱氧野尻霉素-蒙脱土复合物。所制备的1-脱氧野尻霉素-蒙脱土复合物在纯净水、磷酸二氢钠缓冲液(p H 7.4)、0.1 mol·L-1盐酸溶液中均能快速释放,且溶液体积越大,1-脱氧野尻霉素释放率越高。结论钠基蒙脱土对1-脱氧野尻霉素具有缓释效果,可以用于1-脱氧野尻霉素缓释制剂的研究。
Objective To investigate the effect of sodium montmorillonite (Na-MMT) on the release of 1-deoxynojirimycin (DNJ) in mulberry leaves. Method The sodium 1-deoxynojirimycin-montmorillonite composite was prepared by mixing it into the sodium montmorillonite layer with stirring. By calculating the sodium montmorillonite 1-deoxynojirimycin drug loading to select the appropriate adsorption time, temperature, feed ratio, and under the conditions of 1-deoxynojirimycin - montmorillonite composite was In vitro experiments, evaluation of sustained release effect. Results When the amount of sodium montmorillonite was 5.00 g and the amount of mulberry leaf extract was 16.00 g (25.1% 1-deoxynojirimycin), the mixture was stirred in deionized water at 1000 mL for 1 h at 100 ° C 1-deoxynojirimycin-montmorillonite complex was prepared with a drug loading of 111.64 mg · g-1. The prepared 1-deoxynojirimycin-montmorillonite complex can be rapidly released in purified water, sodium dihydrogen phosphate buffer (p H 7.4) and 0.1 mol·L -1 hydrochloric acid solution, and the larger the volume of the solution , 1-deoxynojirimycin release rate is higher. Conclusion sodium montmorillonite has a sustained-release effect on 1-deoxynojirimycin and can be used in the study of 1-deoxynojirimycin sustained-release preparation.