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目的:验证白藜芦醇是否可以抑制胃癌SGC-7901细胞增殖和迁移及其信号通路。方法:用不同浓度白藜芦醇干预SGC-7901细胞,再用LY-294002和IGF-1分别用来抑制和激活Pi3K/AKT通路。MTT法测细胞增殖,划痕试验和Transwell试验测细胞迁移,Western blot检测细胞迁移相关蛋白(MMP-2、MMP-9)、细胞迁移相关蛋白(P21、P27)、以及AKT、p-AKT的表达情况;结果:相比于对照组,白藜芦醇组胃癌细胞增殖和迁移减弱(P=0.001),p-AKT表达减少(P<0.001);LY-294002可以抑制p-AKT的表达(P=0.004),和白藜芦醇一样可以抑制胃癌细胞的增殖和迁移;IGF-1可以显著增加p-AKT的表达(P<0.001),可以逆转白藜芦醇对胃癌细胞增殖和迁移的抑制作用。结论:白藜芦醇通过抑制Pi3K/AKT信号通路抑制胃癌细胞增殖和迁移。
Objective: To verify whether resveratrol can inhibit the proliferation and migration of gastric cancer cell line SGC-7901 and its signaling pathway. METHODS: SGC-7901 cells were treated with different concentrations of resveratrol, and then used to inhibit and activate Pi3K / AKT pathway by LY-294002 and IGF-1 respectively. MTT assay was used to measure cell proliferation, scratch test and Transwell assay. Western blot was used to detect cell migration related proteins (MMP-2, MMP-9), cell migration related proteins (P21, P27) (P = 0.001). The expression of p-AKT was decreased in resveratrol group (P <0.001). LY-294002 could inhibit the expression of p-AKT P = 0.004), and resveratrol could inhibit the proliferation and migration of gastric cancer cells. IGF-1 could significantly increase the expression of p-AKT (P <0.001), and could reverse the effect of resveratrol on the proliferation and migration of gastric cancer cells Inhibition. Conclusion: Resveratrol can inhibit the proliferation and migration of gastric cancer cells through inhibiting Pi3K / AKT signaling pathway.