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建立了超高效液相色谱-串联质谱法测定人血浆中的西酞普兰,并考察其在健康人体内的药动学。采用Venusil MP C18色谱柱分离,以0.05%甲酸溶液∶乙腈(30∶70)为流动相,通过电喷雾离子化四极杆串联质谱,以多反应监测(MRM)方式进行检测,监测离子对m/z 325.22→m/z 109.25(西酞普兰)和m/z 265.23→m/z 167.37(内标盐酸苯海拉明)。西酞普兰的线性范围为0.55~137.04 ng/ml,日内、日间RSD均小于10%。血浆中西酞普兰低、中、高浓度水平的提取回收率分别为(89.6±2.1)%、(72.4±0.6)%和(86.3±2.5)%;相对回收率分别为(92.6±7.0)%、(100.7±7.3)%和(106.4±4.4)%。
The establishment of ultra performance liquid chromatography - tandem mass spectrometry citalopram in human plasma, and investigate its pharmacokinetics in healthy humans. The samples were separated by Venusil MP C18 column and detected by multi-reaction monitoring (MRM) with electrospray ionization quadrupole mass spectrometry using 0.05% formic acid solution: acetonitrile (30:70) as mobile phase. / z 325.22 → m / z 109.25 (citalopram) and m / z 265.23 → m / z 167.37 (internal standard diphenhydramine hydrochloride). Citalopram linear range of 0.55 ~ 137.04 ng / ml, day and day RSD were less than 10%. The recoveries of plasma concentrations of citalopram at low, medium and high levels were (89.6 ± 2.1)%, (72.4 ± 0.6)% and (86.3 ± 2.5)%, respectively. The relative recoveries were 92.6 ± 7.0% (100.7 ± 7.3)% and (106.4 ± 4.4)%, respectively.