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目的观察大鼠脑出血后神经细胞凋亡和蛋白激酶B(AKt)的表达,探讨β-七叶皂甙钠的干预作用及机制。方法将雄性SD大鼠随机分为正常组、假手术组、脑出血组和β-七叶皂甙钠治疗组。后3组又各分为6h、12h、1d、3d、7d组;用Ⅶ型胶原酶注入到大鼠右侧苍白球诱导脑出血模型,采用原位末端标记技术(TUNEL)和免疫组化方法检测损伤侧皮质区细胞凋亡及AKt表达的动态变化以及β-七叶皂甙钠对此的影响。结果脑出血组TUNEL阳性细胞于脑出血后6h出现,AKt于脑出血后12h表达增多,二者均在第3d达到高峰,1周仍有表达。治疗组1d、3d、7d时间点TUNEL阳性细胞较脑出血组明显减少(P<0·05~0·01),AKt表达较脑出血组显著增加(P<0·05~0·01)。结论β-七叶皂甙钠可以减少脑出血后神经细胞的凋亡,其机制可能与上调AKt表达有关。
Objective To observe the neuronal apoptosis and the expression of protein kinase B (AKT) after intracerebral hemorrhage in rats, and to explore the intervention effect and mechanism of β-aescinate. Methods Male SD rats were randomly divided into normal group, sham operation group, intracerebral hemorrhage group and β-aescinate treatment group. The latter three groups were divided into 6h, 12h, 1d, 3d and 7d groups. The rats were injected with type Ⅶ collagenase into the right globus pallidus to induce cerebral hemorrhage. TUNEL and immunohistochemistry To detect the dynamic changes of apoptosis and AKT expression in injured cortex and the effect of β-aescinate on it. Results TUNEL positive cells in ICH group appeared at 6h after intracerebral hemorrhage. The expression of AKt increased at 12h after intracerebral hemorrhage. Both of them reached the peak on the 3rd day and remained in 1 week. TUNEL positive cells in the treatment group were significantly decreased (P <0.05-0.01) at 1 d, 3 d and 7 d, and AKT expression was significantly increased (P <0.05-0.01) than those in the ICH group. Conclusion β-aescinate can reduce the apoptosis of neural cells after intracerebral hemorrhage, which may be related to the up-regulation of AKt expression.