论文部分内容阅读
目的闭塞性细支气管炎(OB)是临床肺移植后主要致死因素且发病机制不清,拟用小鼠气管异位移植模拟OB的病变过程,为研究OB提供动物模型。方法将小鼠分成同基因移植对照组(BALB/C→BALB/C)与异基因移植实验组(BALB/C→C57BL/6)两大组,供体小鼠气管移植到受体小鼠背部两侧皮下,术后3、7、14、21、28 d取出气管移植物,苏木精-伊红染色观察比较组织形态改变,统计分析其上皮覆盖率和管腔闭塞率。结果共完成70例小鼠气管同种异位移植手术,手术成功率94.3%。不同取材时间,同基因对照组和异基因实验组气管移植物苏木精-伊红染色有不同改变,其中以术后21 d实验组气管移植物OB改变最为典型,而对照组气管移植物组织结构基本正常,此时上皮覆盖率两组分别为:0%和(92±6)%,管腔闭塞率两组分别为:(73±12)%和<3%,差异均有显著性意义。结论小鼠气管异位移植成功模拟肺移植后OB的病变过程,术后21 d可作为研究OB的重要时间点。手术操作简便,成功率高,技术稳定。适用于临床肺移植术后OB的发病机制和治疗学的研究。
Objective Obstructive bronchiolitis (OB) is the major lethal factor after clinical lung transplantation and its pathogenesis is unclear. Tracheal xenotransplantation is used to simulate the pathological changes of OB in order to provide animal models for the study of OB. Methods The mice were divided into two groups, the same group (BALB / C → BALB / C) and allogeneic transplantation group (BALB / C → C57BL / 6). The donor mice were transplanted into the back of recipient mice The tracheal grafts were removed subcutaneously and 3, 7, 14, 21 and 28 days after operation. The morphological changes of the trachea were observed by hematoxylin-eosin staining. The epithelial coverage rate and lumen occlusion rate were statistically analyzed. Results A total of 70 tracheal allografts were performed. The successful rate was 94.3%. The tracheal hematoxylin-eosin staining varied with time and different groups of tracheal allografts in the control group and the allogeneic group. The changes of tracheal OB in the experimental group at 21 d after operation were the most typical, while the tracheal graft in the control group The structure was basically normal, the epithelial coverage rate was 0% and (92 ± 6)% respectively, and the rate of lumen occlusion was (73 ± 12)% and <3%, respectively, with significant difference . Conclusion Tracheal xenotransplantation in mice successfully simulated the pathological changes of OB after lung transplantation, and could be used as an important time point for the study of OB after 21 days. Surgical operation is simple, high success rate, technical stability. It is suitable for the study of the pathogenesis and treatment of OB after clinical lung transplantation.