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目的:观察牡丹皮对阑尾脓肿血瘀模型大鼠血清生化、血液流变学、阑尾脓肿湿重和病理形态学的影响,探讨其清热凉血活血消痈作用机制。方法:取阑尾脓肿血瘀模型大鼠50只,分为丹皮高、中、低剂量组、大黄牡丹皮汤组和模型对照组,另取正常及行假手术大鼠各10只,共为7个组。给药组分别给予丹皮提取液16、8、4g/kg,大黄牡丹皮汤药液16 g/kg,对照组给予生理盐水,各组给药体积均为20 mL/kg,连续给药14 d。末次给药后40 min,摘眼球取血,其中2.5 mL加肝素抗凝,测定血液黏度、血沉及红细胞压积。其余血液分离血清,测超氧化物歧化酶(SOD)、丙二醛(MDA)和一氧化氮(NO)。并仔细分离已形成的阑尾脓肿,用电子天平称量湿重。然后用10﹪福尔马林固定,石蜡包埋,经HE染色后作病理检查。结果:与对照组比较,假手术组各指标无明显变化;模型组大鼠血清中SOD活性降低(P<0.01)、MDA和NO含量升高(P<0.01)、全血的高切、低切及血沉均显著升高(P<0.01);与模型组比较,高剂量组能使大鼠血清中SOD含量升高(P<0.05)、MDA和NO的含量降低(P<0.01)、全血高切、低切及血沉显著降低(P<0.01),阑尾脓肿湿重减小(P<0.01),病理组织学病变减轻(P<0.01)。中剂量组和低剂量组对上述指标也有部分改善作用。结论:牡丹皮清热凉血活血消痈的作用机制可能与抑制炎症的发展,降低血液黏度及聚集状态,改善阑尾脓肿大鼠血瘀状态有关。
OBJECTIVE: To observe the effect of Cortex Moutan on serum biochemistry, hemorheology, appendix abscess wet weight and pathomorphology in rats with appendiceal abscess blood stasis, and to explore the mechanism of clearing away heat, cooling blood and promoting blood circulation. Methods: 50 appendiceal abscess blood stasis model rats were divided into high, medium and low doses of Danpi group, the group of rhubarb and moutan soup and control group, another normal and sham rats 10 7 groups. The rats in the treatment group were treated with 16, 4, 4 g / kg of Danpi extract, 16 g / kg of the rhubarb and Moutan liquid respectively, and normal saline in the control group. Each group was administered with a volume of 20 mL / kg and continuous administration of 14 d. 40 min after the last administration, eyeballs were taken for blood sampling, 2.5 mL of heparin was added for anticoagulation, and blood viscosity, erythrocyte sedimentation rate and hematocrit were measured. The remaining blood serum, measured superoxide dismutase (SOD), malondialdehyde (MDA) and nitric oxide (NO). And careful separation of the formed appendix abscess, weighing the wet weight with an electronic balance. Then fixed with 10% formalin, embedded in paraffin, HE stained for pathological examination. Results: Compared with the control group, there was no significant change in each index in the sham operation group. The activity of SOD in the model group decreased (P <0.01), the content of MDA and NO increased (P <0.01) (P <0.01). Compared with the model group, the high dose group could increase the content of SOD in serum (P <0.05) and the content of MDA and NO in the model group Blood high cut, low cut and ESR were significantly decreased (P <0.01). The wet weight of appendix abscess was decreased (P <0.01), and the histopathological lesion was relieved (P <0.01). The middle-dose group and low-dose group also have some improvement on the above indicators. Conclusion: The mechanism of clearing away heat, cooling blood and removing blood circulation of the tree stem may be related to inhibiting the development of inflammation, lowering blood viscosity, aggregating state and improving blood stasis status of appendix abscess rats.