[Objective] The aim of this study was to explore the sterilization effects on Escherichia coli by adding bacterial inhibitor (CaCN2 ) during the process of cattle manure composting so as to provide a theoretical basis for cattle manure harmless treatment. [Method] Both experimental groups supplemented with 2.0% bacterial inhibitor and control groups without bacterial inhibitor were cultured under different temperatures (20, 30, 37, 50, 60 ℃) to determine the optimal composing temperature. Under 30 ℃,different bacterial inhibitor doses (0, 2.0%, 2.5%, 3.0%) were added into the compost to obtain the optimal bacterial inhibitor addition dose. [Result] 30, 50 and 60 ℃ were ideal temperatures for sterilization of E.coli. Under 30 ℃,E.coli couldnt be detected in 2.5% dose group and 3.0% dose group after culture for 48 h, demonstrating no less than 2.5% bacterial inhibitor should be added. [Conclusion] It has an important significance to enhance the sterilization effects on E.coli by adding CaCN2 into cattle manure compost especially in winter.