论文部分内容阅读
目的：了解尿毒症患者透析前后补体活化与荷Ｃ３ｄ免疫复合物（Ｃ３ｄ－ＩＣ）水平。方法：双抗体夹心ＥＬＩＳＡ法。结果：透析后Ｃ３ｄ含量及Ｃ３ｄ－ＩＣ水平较透析前显著下降，而Ｃ３浓度则显著升高，在所有３３例Ｃ３ｄ－ＩＣ阳性者中，有２９例Ｃ３ｄ含量高于正常水平，二者水平变化呈现高度的正相关；而透析前的９例Ｃ３ｄ－ＩＣ阴性者中，Ｃ３ｄ含量亦明显增高。结论：尿毒症患者体内补体的激活除主要由Ｃ３ｄ－ＩＣ引起外，可能还与血清中存在的非免疫性激活物有关。
OBJECTIVE: To investigate the levels of complement activation and C3d-immune complex (C3d-IC) in patients with uremia before and after dialysis. Methods: Double antibody sandwich ELISA. Results: The level of C3d and the level of C3d-IC decreased significantly after dialysis compared with that before dialysis, while the level of C3 increased significantly. Among all 33 patients with C3d-IC positive, 29 patients had higher level of C3d than the normal level Showing a high degree of positive correlation; and dialysis before 9 patients with C3d-IC negative, C3d content was also significantly higher. CONCLUSION: Activation of complement in patients with uremia is mainly caused by C3d-IC, and may be related to the presence of non-immune activators in serum.