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目的探讨miRNA-222(miR-222)在结直肠癌组织中的表达及意义。方法采用实时定量PCR检测44例结直肠癌组织及癌旁正常组织中miR-222的表达水平,分析其与临床病理指标之间关系。结果 miR-222在44例癌组织标本中的表达量为0.0085(0.0041,0.0428),低于癌旁正常组织中的0.0188(0.0120,0.0326)(P<0.01)。miR-222在黏液性腺癌中的表达量为0.1461(0.0478,0.4251),低于非黏液性腺癌的0.4373(0.2012,1.1882)(P<0.05)。miR-222表达与患者的性别、年龄和结直肠癌的分化程度、临床分期、浸润深度、淋巴结和远处转移均无明显相关性(P>0.05)。结论 miR-222参与结直肠癌发生和发展的调控。
Objective To investigate the expression and significance of miRNA-222 (miR-222) in colorectal cancer. Methods Real-time quantitative PCR was used to detect the expression of miR-222 in 44 colorectal cancer tissues and adjacent normal tissues, and its relationship with clinicopathological parameters was analyzed. Results The expression of miR-222 in 44 specimens of cancerous tissues was 0.0085 (0.0041,0.0428), which was lower than that of normal tissues (0.0188, 0.0120,0.0326) (P <0.01). The expression level of miR-222 in mucinous adenocarcinoma was 0.1461 (0.0478, 0.4251), which was lower than that of non-mucinous adenocarcinoma (0.3737) (0.2012,1.1882) (P <0.05). There was no significant correlation between the expression of miR-222 and the gender, age and the degree of differentiation, clinical stage, depth of invasion, lymph node and distant metastasis of colorectal cancer (P> 0.05). Conclusion miR-222 is involved in the development of colorectal cancer.