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僵蚕是传统中药材,筛选对家蚕具有高致病性的球孢白僵菌用于提高规模化生产僵蚕的产量和品质。从蚕区采集的僵蚕虫体分离纯化到一株高致病力的白僵菌,通过形态学鉴定和rRNA ITS序列系统发育分析,确定该菌株为球孢白僵菌(Beauveria bassiana),命名为Beauveria bassiana JC-1。优化该菌株应用于僵蚕生产中的接种菌液浓度为1×106~5×106mL-1,用此浓度的菌液喷洒接种5龄起蚕后5~6 d,幼虫大量死亡,僵化率达90%以上,万头蚕可生产僵蚕约5 kg。利用该菌株感染家蚕收获所得僵蚕的各项质量指标均符合《中国药典》中的有关标准,且僵蚕的总灰分和酸不溶性灰分含量较市售僵蚕更低。初步认为该菌株在僵蚕的标准化、规模化生产中具有应用价值。
Silkworm is a traditional Chinese herbal medicine, screening of silkworm highly pathogenic Beauveria bassiana used to improve the scale production of silkworm production and quality. Isolation and purification of a highly virulent Beauveria bassiana from the silkworm area collected from the silkworm worm, through morphological identification and rDNA ITS sequence phylogenetic analysis to determine the strain Beauveria bassiana (Beauveria bassiana), named Beauveria bassiana JC-1. The inoculum density of this strain was optimized to be 1 × 106 ~ 5 × 106 mL-1. The larvae died after 5 ~ 6 d inoculation with 5-day-old silkworm More than 90%, ten thousand silkworms can produce about 5 kg of silkworm. The quality indicators of the silkworm harvested from the silkworm infected by the strain all met the relevant standards in Chinese Pharmacopoeia, and the total ash content and acid-insoluble ash content of the silkworm silkworm were lower than that of the commercially available silkworm silkworm. It is preliminarily believed that the strain has application value in the standardization and scale production of silkworm silkworm.