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A rapid and sensitive fluorometric method for the enzymatic detection of ethanol using CdSe/ZnS quantum dots (QDs) is proposed.The photoluminescence of QDs is sensitive to H2O2.This finding leads to a novel approach for the determination of ethanol using alcohol oxidase (AOx) which,on oxidation of ethanol,produces H2O2.The method has higher sensitivity,wider analytical range (0.1-8 mmol/L),and a lower detection limit (0.05 mmol/L).The relationship between quenching of the photoluminescence of the QDs and the concentration of ethanol is linear.
A rapid and sensitive fluorometric method for the enzymatic detection of ethanol using CdSe / ZnS quantum dots (QDs) is proposed. The photoluminescence of QDs is sensitive to H2O2. This finding leads to a novel approach for the determination of ethanol using alcohol oxidase (AOx ) which, on oxidation of ethanol, produces H2O2.The method has higher sensitivity, wider analytical range (0.1-8 mmol / L), and a lower detection limit (0.05 mmol / L). The relationship between quenching of the photoluminescence of the QDs and the concentration of ethanol is linear.